Hasta La Motion!

So I use nuke and AE/3d rendering programs a lot and I am just about to reinstall my software because i got a new crucial m4. I know in an ideal world you should be running your OS on one drive, your software on another, be reading input from another, and outputting to another.

With these drives though I am not sure what I should have where. Are the benefits of using separate drives minimal when one drive is so much faster? Should I just install everything on the m4 or should I put win7 on the 80gb drive and install all my software on the m4? Currently I plan on installing the OS and all my software on the M4 and using the 80gb ssd to read from and output to the 2tb drive.

256gb crucial m4: read:415mb/s write 260 mb/s
80gb intel x-25m read:250mb/s write : 70mb/s
2tb 64mb cache HD
1tb 32mb cache HD